Coding

Part:BBa_K762001:Design

Designed by: Menuka Samaranayake   Group: iGEM12_Wisconsin-Madison   (2012-09-24)


TCC Negative control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2327
    Illegal AgeI site found at 2439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 162


Design Notes

Designed purposely to include a stop codon prematurely in the sequence to prevent translation


Source

Based off of UW-Madison's BBa_K762100

References